Subunit structure of chondroitinase ABC from Proteus vulgaris.
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چکیده
منابع مشابه
Recombinant expression, purification, and biochemical characterization of chondroitinase ABC II from Proteus vulgaris.
Chondroitin lyases (or chondroitinases) are a family of enzymes that depolymerize chondroitin sulfate (CS) and dermatan sulfate (DS) galactosaminoglycans, which have gained prominence as important players in central nervous system biology. Two distinct chondroitinase ABC enzymes, cABCI and cABCII, were identified in Proteus vulgaris. Recently, cABCI was cloned, recombinantly expressed, and exte...
متن کاملChondroitinase ABC I from Proteus vulgaris: cloning, recombinant expression and active site identification.
GalAGs (galactosaminoglycans) are one subset of the GAG (glycosaminoglycan) family of chemically heterogeneous polysaccharides that are involved in a wide range of biological processes. These complex biomacromolecules are believed to be responsible for the inhibition of nerve regeneration following injury to the central nervous system. The enzymic degradation of GAG chains in damaged nervous ti...
متن کاملThe degradation of cartilage chondroitin sulphate by the chondroitinase of Proteus vulgaris.
Specificity. Pyruvate is reduced at less than 2 % of the rate of an equimolar amount of hydroxypyruvate by this preparation of the enzyme. Since both pyruvate and hydroxypyruvate rapidly oxidize DPNH in the presence of lactic dehydrogenase of muscle, which is obtainable commercially (Boehringer und Soehne, Mannheim), a differential analysis of pyruvate and hydroxypyruvate in mixtures of the two...
متن کاملSustained Delivery of Chondroitinase ABC from Hydrogel System
In the injured spinal cord, chondroitin sulfate proteoglycans (CSPGs) are the principal responsible of axon growth inhibition and they contribute to regenerative failure, promoting glial scar formation. Chondroitinase ABC (chABC) is known for being able to digest proteoglycans, thus degrading glial scar and favoring axonal regrowth. However, its classic administration is invasive, infection-pro...
متن کاملBacteriophage typing of Proteus mirabilis, Proteus vulgaris, and Proteus morganii.
A bacteriphage typing scheme for differentiating Proteus isolated from clinical specimens was developed. Twenty-one distinct patterns of lysis were seen when 15 bacteriophages isolated on 8 Proteus mirabilis, 1 P. vulgaris, and 1 P. morganii were used to type 162 of 189 (85.7%) P. mirabilis and P. vulgaris isolates. Seven phages isolated on 3 P. morganii were used to type 13 of 19 (68.4%) P. mo...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1986
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.50.1057